Program 1: Different Cell Culture Techniques

Objective:
To build a strong foundation in mammalian and microbial cell culture, equipping participants with essential theoretical knowledge and practical skills.

Core Modules:

1. Aseptic Techniques

• Introduction to sterile work environments
• Use of biosafety cabinets and sterile tools
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2. Media Preparation

• Preparation of complete media for various cell types
• Understanding pH, supplements (FBS, antibiotics), and buffering systems

3. Subculturing Techniques

• Passaging adherent and suspension cells
• Trypsinization, cell splitting ratios, and viability assessment

4. Cell Viability Assays

• Trypan Blue exclusion
• MTT and other metabolic assays

5. Cryopreservation & Thawing

• Freezing protocols using DMSO
• Safe recovery of cell stocks from cryostorage

Ideal For:
Beginners and early-stage researchers looking to master foundational skills in academic, industrial, or clinical laboratory settings.

Program 2: Hematopoietic Stem Cell (HSC) Culture & Analysis

Objective:
To provide comprehensive training in isolating, culturing, analyzing, and characterizing hematopoietic stem cells from blood samples.

Modules:

1. Introduction to Hematopoietic Stem Cells

• Biology of HSCs: origin, differentiation, and role in hematopoiesis
• Importance in regenerative medicine and transplantation

2. Identification of Blood Cell Types

• Use of Romanowsky stains: Leishman, Wright, Giemsa
• Morphological classification under light microscopy

3. Isolation Techniques

• ACK lysis for RBC removal
• Ficoll/Histopaque gradient centrifugation for PBMCs
• EasySep and Percoll for stem cell enrichment

4. HSC Culture Setup

• Use of DMEM or RPMI-1640 with 10–20% FBS and antibiotics
• Seeding in low-adherence plates and maintaining sterility in CO₂ incubators

5. Cell Staining & Microscopic Analysis

• Trypan Blue viability assay
• Giemsa, H&E for morphology
• MTT for cell proliferation/metabolic activity

6. Media Preparation, Subculturing & Cryopreservation

• Media formulation for HSCs
• Gentle subculturing of suspension cells
• Long-term storage using cryovials and DMSO cryoprotectants

7. Molecular Biology Integration

• Genomic DNA extraction from cultured cells
• Agarose gel electrophoresis for DNA analysis
• Visualization using a gel documentation system

8. Scientific Data Analysis & Reporting

• Compilation and interpretation of experimental results
• Cell counting (manual and automated)
• Preparation of lab reports and scientific presentations

Ideal For:
Advanced undergraduates, postgraduates, and lab professionals working in hematology, stem cell research, or clinical diagnostics.

https://hereditybio.in/blog/learn-culture-discover-new-hands-on-cell-culture-training-batches-at-herrick-healthcare-2/