Course Title: Plant Biotechnology and Molecular Biology - Hands-On Training
Duration: 3 Months
Program Highlights:
This comprehensive course is designed to provide students with a thorough understanding of plant biotechnology and molecular biology techniques. Participants will gain practical experience and theoretical knowledge essential for research and development in the field.
Modules Overview:
Module 1: Phytocompounds Extraction
- Objective: Learn techniques for extracting bioactive compounds from plants.
- Collection of Herbal Plant Samples: Identification of suitable species and proper sample collection.
- Plant Sample Processing: Techniques for preparing fresh material, including drying and grinding.
- Crude Extraction Techniques: Methods such as Soxhlet extraction and cold maceration.
- Secondary Metabolites Extraction: Isolate flavonoids, alkaloids, and terpenoids.
- Phytochemical Analysis: Conduct qualitative and quantitative analysis of extracts.
Module 2: In-Vitro Bio-assays of Phytocompounds
- Objective: Evaluate the biological activity of extracted phytocompounds.
- Phytocompounds Extract Preparation: Standardization of extract concentrations.
- Anti-bacterial Activity: Assess efficacy using disc diffusion and broth dilution methods.
- Anti-fungal Activity: Techniques to test antifungal properties against pathogens.
- Anti-candidal Activity: Evaluate extracts against Candida species.
- Anti-oxidant Activity by DPPH Method: Application of the DPPH assay to determine antioxidant capacity.
Module 3: Nanomaterial Synthesis
- Objective: Explore green synthesis methods for producing nanoparticles.
- Plant Sample Processing: Preparation of extracts for nanoparticle synthesis.
- Green Synthesis of Nanoparticles: Principles and benefits of using green chemistry.
- Synthesis of Silver Nanoparticles Using Neem Leaf Extract: Methodology and analysis.
- Synthesis of Zinc Oxide Nanoparticles Using Aloe Vera Gel: Protocol for extraction and characterization.
- Synthesis of Copper Nanoparticles Using Mint Extract: Step-by-step synthesis and applications.
Module 4: Bio-active Compounds Extraction
- Objective: Refine techniques for extracting and analyzing bioactive compounds.
- Reagents and Stock Solution Preparations: Preparation of solutions for extraction.
- Advanced Extraction Techniques: Crude extraction methods focused on specific classes of compounds.
- UV-VIS Analysis: Use UV-VIS spectroscopy for compound identification.
- Anti-bacterial Activity of Crude Extract: Conduct bioassays to evaluate extract efficacy.
- Minimum Inhibitory Concentration (MIC) Test: Determine effectiveness against bacterial strains.
Module 5: Molecular Biology
- Objective: Apply molecular biology techniques in plant research.
- Isolation of Plant DNA: Techniques for extracting high-quality DNA from plant tissues.
- PCR Techniques for Plant Pathogens: Apply PCR for detection and characterization.
- cDNA Synthesis: Extraction protocols for genomic and complementary DNA.
- Gene Expression Analysis: Methods for assessing gene expression levels in transgenic plants.
- Gel Electrophoresis of Plant DNA Fragments: Separation and visualization of PCR products.
Module 6: Chromatographic Techniques
- Objective: Learn chromatography methods for separating plant pigments and compounds.
- Separation of Plant Pigments Using Paper Chromatography: Techniques for analyzing pigment composition.
- Analyzing Leaf Extracts by Thin Layer Chromatography (TLC): Application of TLC for various extracts.
- Chromatographic Separation of Chlorophyll and Carotenoids: Techniques specific to spinach extracts.
- Identifying Plant Pigments in Flower Petals Using Chromatography: Analyze pigments in ornamental plants.
Call to Action:
Join our hands-on training program in Plant Biotechnology and Molecular Biology at Heredity Biosciences! Enhance your skills, gain valuable practical experience, and prepare for a successful career in research and development. Enroll today and take the first step towards a rewarding future in the life sciences! Visit our website for more information and registration.
Course Title: Plant Biotechnology and Molecular Biology - Hands-On Training
Duration: 3 Months
Program Highlights:
This course is designed to equip students with a comprehensive understanding of plant biotechnology and molecular biology techniques. Participants will engage in hands-on training that combines theoretical knowledge with practical applications essential for careers in research and development.
Modules Overview:
Module 1: Phytocompounds Extraction
- Objective: Master techniques for extracting bioactive compounds from plants.
- Collection of Herbal Plant Samples: Identify suitable species and learn proper collection and preservation methods.
- Plant Sample Processing: Techniques for preparing fresh material, including drying and grinding.
- Crude Extraction Techniques: Implement solvent extraction methods (e.g., Soxhlet extraction, cold maceration).
- Secondary Metabolites Extraction: Isolate flavonoids, alkaloids, and terpenoids using various extraction methods.
- Phytochemical Analysis: Conduct qualitative and quantitative analysis of plant extracts to determine phytoconstituents.
Module 2: In-Vitro Bio-assays of Phytocompounds
- Objective: Evaluate the biological activity of extracted phytocompounds.
- Phytocompounds Extract Preparation: Standardize extract concentrations for bioassays.
- Anti-bacterial Activity: Assess antibacterial efficacy through disc diffusion and broth dilution methods.
- Anti-fungal Activity: Test antifungal properties against various pathogens.
- Anti-candidal Activity: Evaluate extracts against Candida species.
- Anti-oxidant Activity by DPPH Method: Determine the antioxidant capacity of the extracts using the DPPH assay.
Module 3: Nanomaterial Synthesis
- Objective: Explore green synthesis methods for producing nanoparticles.
- Plant Sample Processing: Prepare plant extracts suitable for nanoparticle synthesis.
- Principles of Green Synthesis: Understand the benefits of using green chemistry in nanotechnology.
- Synthesis of Silver Nanoparticles Using Neem Leaf Extract: Methodology for synthesis and analysis of nanoparticles.
- Synthesis of Zinc Oxide Nanoparticles Using Aloe Vera Gel: Protocol for extraction and characterization.
- Green Synthesis of Copper Nanoparticles Using Mint Extract: Detailed steps for synthesis and applications.
Module 4: Bio-active Compounds Extraction
- Objective: Refine techniques for extracting and analyzing bioactive compounds.
- Preparation of Solutions for Extraction: Learn how to prepare reagents and stock solutions.
- Advanced Extraction Techniques: Focus on isolating specific classes of bioactive compounds.
- UV-VIS Analysis: Use UV-VIS spectroscopy for identifying compounds.
- Anti-bacterial Activity of Crude Extract: Conduct bioassays to evaluate the efficacy of extracts.
- Minimum Inhibitory Concentration (MIC) Test: Determine the effectiveness of extracts against bacterial strains.
Module 5: Molecular Biology
- Objective: Apply molecular biology techniques in plant research.
- Isolation of Plant DNA: Techniques for high-quality DNA extraction from plant tissues.
- PCR Techniques for Plant Pathogens: Use PCR for detection and characterization of pathogens.
- cDNA Synthesis: Protocols for genomic and complementary DNA extraction.
- Gene Expression Analysis: Methods for assessing gene expression in transgenic plants.
- Gel Electrophoresis of Plant DNA Fragments: Techniques for separation and visualization of PCR products.
Module 6: Chromatographic Techniques
- Objective: Learn chromatography methods for separating plant pigments and compounds.
- Separation of Plant Pigments Using Paper Chromatography: Techniques for analyzing pigment composition in plants.
- Analyzing Leaf Extracts by Thin Layer Chromatography (TLC): Application of TLC to various plant extracts.
- Chromatographic Separation of Chlorophyll and Carotenoids: Methods specific to spinach and other plants.
- Identifying Plant Pigments in Flower Petals Using Chromatography: Analyze pigments in ornamental plants for research applications.
Conclusion:
The Plant Biotechnology and Molecular Biology program at Heredity Biosciences provides students with the essential skills and knowledge to excel in research and industry. By engaging in practical experiences, students will be well-prepared to contribute to advancements in biotechnology and molecular biology.
Call to Action:
Join our hands-on training program today and take your first step towards a rewarding career in Plant Biotechnology and Molecular Biology! Visit our website for more information and registration details.
Q: Who is this hands-on training program intended for?
A: This program is designed for students and professionals in the fields of biotechnology, molecular biology, plant sciences, and life sciences, including BSc, MSc, M.Tech, and PhD candidates.
Q: What topics will be covered in the training?
A: The training covers various topics such as plant tissue culture, DNA/RNA extraction, PCR techniques, gene expression analysis, and applications of molecular markers in plant breeding.
Q: Do I need prior experience in plant biotechnology or molecular biology to enroll?
A: While some foundational knowledge in these areas is beneficial, it is not mandatory. The program is structured to accommodate learners at various skill levels.
Q: How long is the training program?
A: The hands-on training program typically lasts for 3 months, combining theoretical lectures and practical sessions.
Q: Will there be practical sessions included?
A: Yes, the program includes extensive hands-on training, allowing participants to practice essential techniques in plant biotechnology and molecular biology.
Q: Are materials and equipment provided for the training?
A: Yes, all necessary materials, reagents, and equipment will be provided during the training sessions.
Q: Will participants receive a certificate upon completion?
A: Yes, participants will receive a certificate of completion after successfully finishing the training program.
Q: Is there a research component included in the training?
A: Yes, participants will have opportunities to engage in research projects related to plant biotechnology and can discuss their findings.
Q: How can I apply for the training program?
A: Interested candidates can apply by visiting our website or contacting us directly for more information on the enrollment process.
Q: Are accommodation options provided during the training?
A: Accommodation may be available near the training center. Please connect with our team for further details.
Q: Does Heredity Biosciences offer placement assistance after the training?
A: Yes, we provide placement assistance and support to help graduates explore career opportunities in plant biotechnology and molecular biology.
Q: Where will the training be conducted?
A: The training will take place at our facility located at Heredity Biosciences, Bhubaneswar, Odisha.
For more details, connect with us at our website: Heredity Biosciences
