#recombinantDNAtechnology

Molecular Cloning and Recombinant DNA Technology

Join our comprehensive hands-on training program in Molecular Cloning and Recombinant DNA Technology at Heredity Biosciences. This course is designed for students seeking practical experience in the latest molecular biology techniques.

Participants will learn essential skills such as DNA extraction, restriction enzyme digestion, ligation, and transformation of plasmids into host cells. You will gain proficiency in various cloning strategies and utilize modern techniques for gene expression analysis. Additionally, hands-on sessions will cover gel electrophoresis for analyzing recombinant DNA and assessing cloning success.

This training provides a solid foundation in molecular cloning, equipping you with the expertise needed for a successful career in biotechnology and genetic research. Don’t miss this opportunity to enhance your practical skills and understanding of recombinant DNA technology!

Are you ready to enhance your expertise in Molecular Cloning and Recombinant DNA Technology?

Enroll now in our comprehensive hands-on training program at Heredity Biosciences! This is your opportunity to gain invaluable skills, engage in practical learning, and advance your career in the rapidly evolving fields of microbiology and genetics.

Don’t miss out!

Secure your spot today by visiting our website or contacting us for more information. Join us and take the first step towards mastering essential techniques that can make a significant impact in healthcare and research!

Duration: 3 Months

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Course Details

Course Title: Advanced Molecular Biology Techniques

Course Duration: 3 Months


Aim:

To provide participants with comprehensive hands-on training in molecular biology techniques while facilitating interactions with experts in the field.


Part 1: DNA Techniques

  1. DNA Extraction from Human Blood:

    • Learn techniques for isolating high-quality DNA from human blood samples.
    • Understand the importance of DNA integrity for downstream applications.
  2. DNA Extraction from Pathological Bacteria:

    • Methods for extracting DNA from pathogenic bacterial cultures.
    • Focus on protocols that ensure contamination-free results.
  3. DNA Extraction from Tissues:

    • Techniques for obtaining DNA from various tissue types.
    • Discussion of factors affecting DNA yield and quality.
  4. Agarose Electrophoresis:

    • Set up and run agarose gels to visualize DNA.
    • Analyze DNA size and integrity post-extraction.
  5. Primer Designing Using Bioinformatics Tools:

    • Introduction to bioinformatics software for primer design.
    • Criteria for designing effective primers for PCR applications.

Part 2: RNA Techniques

  1. RNA Isolation from Human Blood:

    • Techniques for extracting RNA from blood samples, ensuring RNA integrity and purity.
  2. Agarose Formaldehyde Gel Preparation for RNA Electrophoresis:

    • Learn to prepare and run agarose formaldehyde gels for RNA visualization.
  3. First Strand cDNA Synthesis:

    • Understand the process of synthesizing complementary DNA (cDNA) from RNA templates.
  4. Optimization of PCR Parameters (DNA and RNA):

    • Techniques for optimizing PCR conditions for both DNA and RNA targets.
  5. Gene Expression Analysis of Different Primers:

    • Analyze gene expression levels using quantitative PCR with designed primers.
  6. Extraction & Purification of Amplified DNA from Agarose Gels Using Binding Matrix Methods:

    • Learn methods for isolating and purifying DNA fragments from agarose gels for downstream applications.

Part 3: Molecular Cloning Techniques

  1. Molecular Cloning:

    • Overview of molecular cloning principles and applications in research.
  2. Cultivation of DH5 Alpha Cells and Competent Cell Preparation:

    • Techniques for growing DH5 alpha cells and preparing competent cells for transformation.
  3. Cultivation of pGEM-T Easy Vector Bearing Bacterial Strain:

    • Understanding vector systems and their role in molecular cloning.
  4. DNA Ligation:

    • Techniques for ligating DNA fragments into vectors.
  5. Bacterial Transformation and Blue-White Screening:

    • Methods for transforming competent cells with recombinant plasmids and selecting successfully transformed colonies.
  6. Plasmid Isolation:

    • Techniques for isolating plasmid DNA from bacterial cultures.
  7. Restriction Digestion of pGEM-T Easy Vector Using Restriction Enzyme:

    • Understanding restriction enzyme digestion and its applications in cloning.
  8. Colony PCR:

    • Perform PCR directly on bacterial colonies to verify the presence of insert DNA in plasmids.

Certification:

Participants will receive a certificate of completion upon successfully finishing the program, demonstrating their expertise in advanced molecular biology techniques.


This curriculum is designed to provide a solid foundation in molecular biology, equipping participants with practical skills essential for research and clinical applications.

Curriculum

FAQ

Certificate

  • Upon completing a course, you may receive a certification or a certificate of completion. This document typically validates your participation and successful completion of the course requirements.

  • Certification serves as evidence of your newly acquired skills or knowledge in a particular subject area and can be valuable for career advancement or further education.

  • Depending on the course provider or institution, the certification may vary in format, recognition, and credibility.

Student Testimonial

We offers training programs in Clinical Microbiology and genetics, Clinical nanotechnology and genetics, Molecular Genetics, Food microbiology and Genetics - both for Life Sciences graduates as well as Medical Professionals.

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