Course Title: Advanced Molecular Biology Techniques
Course Duration: 3 Months
Aim:
To provide participants with comprehensive hands-on training in molecular biology techniques while facilitating interactions with experts in the field.
Part 1: DNA Techniques
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DNA Extraction from Human Blood:
- Learn techniques for isolating high-quality DNA from human blood samples.
- Understand the importance of DNA integrity for downstream applications.
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DNA Extraction from Pathological Bacteria:
- Methods for extracting DNA from pathogenic bacterial cultures.
- Focus on protocols that ensure contamination-free results.
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DNA Extraction from Tissues:
- Techniques for obtaining DNA from various tissue types.
- Discussion of factors affecting DNA yield and quality.
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Agarose Electrophoresis:
- Set up and run agarose gels to visualize DNA.
- Analyze DNA size and integrity post-extraction.
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Primer Designing Using Bioinformatics Tools:
- Introduction to bioinformatics software for primer design.
- Criteria for designing effective primers for PCR applications.
Part 2: RNA Techniques
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RNA Isolation from Human Blood:
- Techniques for extracting RNA from blood samples, ensuring RNA integrity and purity.
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Agarose Formaldehyde Gel Preparation for RNA Electrophoresis:
- Learn to prepare and run agarose formaldehyde gels for RNA visualization.
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First Strand cDNA Synthesis:
- Understand the process of synthesizing complementary DNA (cDNA) from RNA templates.
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Optimization of PCR Parameters (DNA and RNA):
- Techniques for optimizing PCR conditions for both DNA and RNA targets.
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Gene Expression Analysis of Different Primers:
- Analyze gene expression levels using quantitative PCR with designed primers.
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Extraction & Purification of Amplified DNA from Agarose Gels Using Binding Matrix Methods:
- Learn methods for isolating and purifying DNA fragments from agarose gels for downstream applications.
Part 3: Molecular Cloning Techniques
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Molecular Cloning:
- Overview of molecular cloning principles and applications in research.
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Cultivation of DH5 Alpha Cells and Competent Cell Preparation:
- Techniques for growing DH5 alpha cells and preparing competent cells for transformation.
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Cultivation of pGEM-T Easy Vector Bearing Bacterial Strain:
- Understanding vector systems and their role in molecular cloning.
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DNA Ligation:
- Techniques for ligating DNA fragments into vectors.
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Bacterial Transformation and Blue-White Screening:
- Methods for transforming competent cells with recombinant plasmids and selecting successfully transformed colonies.
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Plasmid Isolation:
- Techniques for isolating plasmid DNA from bacterial cultures.
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Restriction Digestion of pGEM-T Easy Vector Using Restriction Enzyme:
- Understanding restriction enzyme digestion and its applications in cloning.
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Colony PCR:
- Perform PCR directly on bacterial colonies to verify the presence of insert DNA in plasmids.
Certification:
Participants will receive a certificate of completion upon successfully finishing the program, demonstrating their expertise in advanced molecular biology techniques.
This curriculum is designed to provide a solid foundation in molecular biology, equipping participants with practical skills essential for research and clinical applications.
Course Title: Molecular Cloning and Recombinant DNA Technology
Course Duration: 3 Months
Course Overview:
This comprehensive curriculum is designed to equip students and professionals with practical and theoretical knowledge of molecular cloning and recombinant DNA technology. Participants will engage in hands-on training, covering various techniques essential for cloning, manipulating, and analyzing DNA.
Module 1: Introduction to Molecular Cloning
Module 2: Vector Systems
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Types of Vectors:
- Overview of plasmids, phagemids, cosmids, and BACs.
- Selection of appropriate vectors for cloning.
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Vector Construction:
- Methods for constructing vectors (e.g., pGEM-T Easy Vector).
- Insertion sites and features of cloning vectors.
Module 3: DNA Manipulation Techniques
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Restriction Enzymes:
- Understanding the role of restriction enzymes in cloning.
- Practical sessions on enzyme digestion of DNA.
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Ligation:
- Techniques for ligating DNA fragments into vectors.
- Optimizing ligation conditions for successful cloning.
Module 4: Transformation Techniques
Module 5: Verification of Clones
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Colony PCR:
- Performing PCR directly on bacterial colonies to verify insert presence.
- Gel electrophoresis analysis of PCR products.
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Restriction Analysis:
- Performing restriction digestion to confirm successful ligation.
- Interpretation of results.
Module 6: Advanced Cloning Techniques
Module 7: Applications of Recombinant DNA Technology
Module 8: Ethics and Safety in Molecular Cloning
- Biosafety Regulations:
- Understanding ethical considerations and regulations in genetic engineering.
- Best practices for laboratory safety and waste disposal.
Assessment and Certification:
- Practical Examination: Assessment of skills in molecular cloning techniques.
- Written Examination: Evaluation of theoretical knowledge of recombinant DNA technology.
- Certification of Completion: Participants will receive a certificate upon successful completion of the program.
This curriculum provides a thorough grounding in molecular cloning and recombinant DNA technology, ensuring participants develop the skills necessary for research and applications in biotechnology.
Q: Who is this hands-on training program intended for?
A: This program is designed for students and professionals in biotechnology, molecular biology, microbiology, and life sciences, including MSc, M.Tech, PhD candidates, and graduates seeking practical experience.
Q: What topics will be covered in the training?
A: The training covers various topics, including vector systems, DNA manipulation techniques, bacterial transformation, verification of clones, advanced cloning techniques, and applications of recombinant DNA technology.
Q: Do I need prior experience in molecular biology to enroll?
A: While some foundational knowledge in molecular biology is beneficial, it is not mandatory. The program is structured to accommodate learners at different skill levels.
Q: How long is the training program?
A: The hands-on training program typically lasts for 3 months, consisting of both theoretical and practical sessions.
Q: Will there be practical sessions included?
A: Yes, the program includes extensive hands-on training where participants will practice molecular cloning techniques and recombinant DNA applications.
Q: Are materials and equipment provided for the training?
A: Yes, all necessary materials, reagents, and equipment will be provided during the training sessions.
Q: Will participants receive a certificate upon completion?
A: Yes, participants will receive a certificate of completion after successfully finishing the training program.
Q: Is there a research component included in the training?
A: Yes, participants will have opportunities to engage in research projects related to molecular cloning and can discuss their findings.
Q: How can I apply for the training program?
A: Interested candidates can apply by visiting our website or contacting us directly for more information on the enrollment process.
Q: Are accommodation options provided during the training?
A: Accommodation may be available near the training center. Please connect with our team for further details.
Q: Does Heredity Biosciences offer placement assistance after the training?
A: Yes, we provide placement assistance and support to help graduates explore career opportunities in molecular biology and biotechnology.
Q: Where will the training be conducted?
A: The training will take place at our facility located at Heredity Biosciences, Bhubaneswar, Odisha.
For more details, connect with us at our website: Heredity Biosciences