Course Title: Advanced Molecular Biology Techniques

Course Duration: 3 Months

Aim:

To provide participants with comprehensive hands-on training in molecular biology techniques while facilitating interactions with experts in the field.

Part 1: DNA Techniques

1. DNA Extraction from Human Blood:

   • Learn techniques for isolating high-quality DNA from human blood samples.
   • Understand the importance of DNA integrity for downstream applications.

2. DNA Extraction from Pathological Bacteria:

   • Methods for extracting DNA from pathogenic bacterial cultures.
   • Focus on protocols that ensure contamination-free results.

3. DNA Extraction from Tissues:

   • Techniques for obtaining DNA from various tissue types.
   • Discussion of factors affecting DNA yield and quality.

4. Agarose Electrophoresis:

   • Set up and run agarose gels to visualize DNA.
   • Analyze DNA size and integrity post-extraction.

5. Primer Designing Using Bioinformatics Tools:

   • Introduction to bioinformatics software for primer design.
   • Criteria for designing effective primers for PCR applications.

Part 2: RNA Techniques

1. RNA Isolation from Human Blood:

   • Techniques for extracting RNA from blood samples, ensuring RNA integrity and purity.

2. Agarose Formaldehyde Gel Preparation for RNA Electrophoresis:

   • Learn to prepare and run agarose formaldehyde gels for RNA visualization.

3. First Strand cDNA Synthesis:

   • Understand the process of synthesizing complementary DNA (cDNA) from RNA templates.

4. Optimization of PCR Parameters (DNA and RNA):

   • Techniques for optimizing PCR conditions for both DNA and RNA targets.

5. Gene Expression Analysis of Different Primers:

   • Analyze gene expression levels using quantitative PCR with designed primers.

6. Extraction & Purification of Amplified DNA from Agarose Gels Using Binding Matrix Methods:

   • Learn methods for isolating and purifying DNA fragments from agarose gels for downstream applications.

Part 3: Molecular Cloning Techniques

1. Molecular Cloning:

   • Overview of molecular cloning principles and applications in research.

2. Cultivation of DH5 Alpha Cells and Competent Cell Preparation:

   • Techniques for growing DH5 alpha cells and preparing competent cells for transformation.

3. Cultivation of pGEM-T Easy Vector Bearing Bacterial Strain:

   • Understanding vector systems and their role in molecular cloning.

4. DNA Ligation:

   • Techniques for ligating DNA fragments into vectors.

5. Bacterial Transformation and Blue-White Screening:

   • Methods for transforming competent cells with recombinant plasmids and selecting successfully transformed colonies.

6. Plasmid Isolation:

   • Techniques for isolating plasmid DNA from bacterial cultures.

7. Restriction Digestion of pGEM-T Easy Vector Using Restriction Enzyme:

   • Understanding restriction enzyme digestion and its applications in cloning.

8. Colony PCR:

   • Perform PCR directly on bacterial colonies to verify the presence of insert DNA in plasmids.

Certification:

Participants will receive a certificate of completion upon successfully finishing the program, demonstrating their expertise in advanced molecular biology techniques.

This curriculum is designed to provide a solid foundation in molecular biology, equipping participants with practical skills essential for research and clinical applications.

Course Title: Molecular Cloning and Recombinant DNA Technology

Course Duration: 3 Months

Course Overview:

This comprehensive curriculum is designed to equip students and professionals with practical and theoretical knowledge of molecular cloning and recombinant DNA technology. Participants will engage in hands-on training, covering various techniques essential for cloning, manipulating, and analyzing DNA.

Module 1: Introduction to Molecular Cloning

• Overview of Molecular Cloning:

  • Understanding the principles and applications of molecular cloning.
  • History and significance of recombinant DNA technology.

• Basic Techniques:

  • Isolation and purification of nucleic acids.
  • Preparation of competent cells.

Module 2: Vector Systems

• Types of Vectors:

  • Overview of plasmids, phagemids, cosmids, and BACs.
  • Selection of appropriate vectors for cloning.

• Vector Construction:

  • Methods for constructing vectors (e.g., pGEM-T Easy Vector).
  • Insertion sites and features of cloning vectors.

Module 3: DNA Manipulation Techniques

• Restriction Enzymes:

  • Understanding the role of restriction enzymes in cloning.
  • Practical sessions on enzyme digestion of DNA.

• Ligation:

  • Techniques for ligating DNA fragments into vectors.
  • Optimizing ligation conditions for successful cloning.

Module 4: Transformation Techniques

• Bacterial Transformation:

  • Methods for transforming competent cells with recombinant DNA.
  • Electroporation and heat shock methods.

• Selection of Transformed Cells:

  • Screening transformed bacteria using antibiotic resistance and blue-white screening.

Module 5: Verification of Clones

• Colony PCR:

  • Performing PCR directly on bacterial colonies to verify insert presence.
  • Gel electrophoresis analysis of PCR products.

• Restriction Analysis:

  • Performing restriction digestion to confirm successful ligation.
  • Interpretation of results.

Module 6: Advanced Cloning Techniques

• Site-Directed Mutagenesis:

  • Techniques for introducing specific mutations into DNA sequences.
  • Applications in protein engineering.

• Gene Expression Cloning:

  • Strategies for expressing cloned genes in prokaryotic and eukaryotic systems.
  • Use of expression vectors and fusion tags.

Module 7: Applications of Recombinant DNA Technology

• Production of Recombinant Proteins:

  • Methods for producing and purifying recombinant proteins.
  • Applications in medicine, agriculture, and industry.

• Gene Therapy and Biotechnology:

  • Overview of gene therapy concepts and applications.
  • Role of recombinant DNA technology in biopharmaceuticals.

Module 8: Ethics and Safety in Molecular Cloning

• Biosafety Regulations:
  • Understanding ethical considerations and regulations in genetic engineering.
  • Best practices for laboratory safety and waste disposal.

Assessment and Certification:

• Practical Examination: Assessment of skills in molecular cloning techniques.
• Written Examination: Evaluation of theoretical knowledge of recombinant DNA technology.
• Certification of Completion: Participants will receive a certificate upon successful completion of the program.

This curriculum provides a thorough grounding in molecular cloning and recombinant DNA technology, ensuring participants develop the skills necessary for research and applications in biotechnology.

Q: Who is this hands-on training program intended for?
A: This program is designed for students and professionals in biotechnology, molecular biology, microbiology, and life sciences, including MSc, M.Tech, PhD candidates, and graduates seeking practical experience.

Q: What topics will be covered in the training?
A: The training covers various topics, including vector systems, DNA manipulation techniques, bacterial transformation, verification of clones, advanced cloning techniques, and applications of recombinant DNA technology.

Q: Do I need prior experience in molecular biology to enroll?
A: While some foundational knowledge in molecular biology is beneficial, it is not mandatory. The program is structured to accommodate learners at different skill levels.

Q: How long is the training program?
A: The hands-on training program typically lasts for 3 months, consisting of both theoretical and practical sessions.

Q: Will there be practical sessions included?
A: Yes, the program includes extensive hands-on training where participants will practice molecular cloning techniques and recombinant DNA applications.

Q: Are materials and equipment provided for the training?
A: Yes, all necessary materials, reagents, and equipment will be provided during the training sessions.

Q: Will participants receive a certificate upon completion?
A: Yes, participants will receive a certificate of completion after successfully finishing the training program.

Q: Is there a research component included in the training?
A: Yes, participants will have opportunities to engage in research projects related to molecular cloning and can discuss their findings.

Q: How can I apply for the training program?
A: Interested candidates can apply by visiting our website or contacting us directly for more information on the enrollment process.

Q: Are accommodation options provided during the training?
A: Accommodation may be available near the training center. Please connect with our team for further details.

Q: Does Heredity Biosciences offer placement assistance after the training?
A: Yes, we provide placement assistance and support to help graduates explore career opportunities in molecular biology and biotechnology.

Q: Where will the training be conducted?
A: The training will take place at our facility located at Heredity Biosciences, Bhubaneswar, Odisha.

For more details, connect with us at our website: Heredity Biosciences