Plant Material Grinding:
- Grind plant material into a fine powder using a mortar and pestle or grinder to facilitate phytochemical extraction.
Phytochemical Extraction:
- Place the powdered plant material in a thimble and extract it with a suitable solvent (e.g., ethanol, methanol, or water).
- Utilize the Soxhlet extraction method to continuously extract phytochemicals from the plant material.
- Collect the concentrated extract in a receiving flask attached to the Soxhlet apparatus.
- Evaporate the solvent under reduced pressure using a rotary evaporator to obtain the crude phytochemical extract.
- Preliminary identification of phytochemicals in the extract, including amino acids, proteins, alkaloids, triterpenoids, flavonoids, and phenols.
Inoculation with Microbes:
- Inoculate plants with beneficial microbes (e.g., rhizobacteria, mycorrhizae) and pathogenic microbes to study their effects on plant health and disease resistance.
- Monitoring Plant Health:
- Monitor plant health and disease symptoms over time, recording changes in growth, vigor, and disease severity.
Evaluation of Microbial Inoculants:
- Evaluate the impact of microbial inoculants on enhancing plant resistance to pathogens by comparing disease symptoms and growth parameters between inoculated and non-inoculated plants.
Biocontrol Using Antagonistic Microbes:
- Isolate antagonistic microbes (e.g., Trichoderma spp., Bacillus spp.) from soil or plant rhizosphere.
- Assess the antagonistic activity of isolated microbes against plant pathogens in vitro and in planta.
- Investigate mechanisms of action involved in biocontrol, such as competition for nutrients or production of antimicrobial compounds.
Pathogen Inhibition Assays:
- Perform in vitro assays to determine the inhibitory effect of antagonistic microbes on the growth of plant pathogens using dual culture or agar diffusion methods.
Molecular methods
DNA Extraction:
- Isolate DNA from plant tissues, microbial cultures, or environmental samples using appropriate extraction methods.
PCR (Polymerase Chain Reaction):
- Amplify specific DNA sequences of interest, such as microbial genes involved in pathogenesis or plant defense responses.
- Perform quantitative PCR (qPCR) to quantify gene expression levels and assess changes in gene expression during interactions.
Sequencing:
- Sequence amplified DNA fragments to determine their nucleotide sequence and identify genetic variations or functional elements.
Gene Expression Analysis:
- Perform reverse transcription PCR (RT-PCR) to analyze gene expression levels of specific plant or microbial genes.
Q. What is the qualification required to enroll in the program?
Ans. Any Life sciences Candidates join the program (BSc, MSc, M.Tech, MBBS, Nursing, and Pharmacy)
Q. Can I enroll in both internship and dissertation training in your institute?
Ans. Yes, you will. During joining the enrolment form. We will provide two certificates.
Q. Do you provide internships to B.sc /M.sc Biotechnology/Microbiology graduates?
Ans- Yes we are providing to all. Kindly visit our website at https://hereditybio.in/training or connect with us at 7381298980.
Q. Can a biotechnology undergraduate do an internship here? If yes what is the process of applying?
Ans-Yes, Kindly connect our team on-7381298980 or visit our website at https://hereditybio.in/.
Q. Does Heredity Bioscience provide placement to the students..?
Ans- We provide 100% placement assistance. The company has exclusive and non-exclusive tie-ups with various companies. All the programs we are offering, either short-term or long-duration projects are industry-oriented and are designed as per requirements of Industries. Please visit https://hereditybio.in/placement
Q. How we can apply for Dissertation/Project work at Heredity Bioscience.?
Ans. Please share your CV with hereditybioacademy@gmail.com or Whatsapp to our Heredity Bioscience team at -7381298980.
Q: What are the main training programs for B.sc /M.sc Life Sciences Students?
Ans. Food Microbiology, Clinical Microbiology, Nanotechnology, Bioinformatics, and many more.
Q: Is there any accommodation provided by the company during the Internships?
Ans- The hostels are near the Academy, our executive will provide you with the details.
Q: How can I pay for my enrolment?
Ans: When we will receive mail confirmation candidates' side? We will generate an offer letter and share it with your registered mail ID.
Q. Do you provide any certificate after completion of the training program?
Ans. Yes, we will provide for certificate after completion of the program.
Q. How many centers do you have?
Ans. We have only one centre at Plot No:818, Mayfair Lagoon, Jayadev Vihar, Bhubaneswar, Odisha. For more details connect us at our website https://hereditybio.in/.
