“Because real scientists don’t just read about cells — they grow them.”

Introduction

Cell culture is the beating heart of modern life science. It’s where discoveries begin — from understanding diseases to developing life-saving drugs and stem-cell-based therapies.
To empower young minds in this transformative field, Heredity Biosciences, in collaboration with Herrick Healthcare Pvt. Ltd., proudly announces two new hands-on cell culture training programs designed for students and early-career researchers.

New batches starting very soon — limited seats available!

Program 1: Different Cell Culture Techniques

“From Tissue to Technology — Mastering the Living Laboratory”

Cell culture is more than growing cells — it’s the art of bringing life into a controlled environment.
This hands-on training program takes you through every stage of tissue-based cell growth, from the moment tissue is excised to when cells thrive under the microscope — integrating both classical culture techniques and molecular biology applications.

What You’ll Learn

Introduction to Cell Culture

• Learn the difference between Primary Cultures, Continuous (Immortalized) Cultures, Adherent Cultures, and Suspension Cultures.
• Understand the importance of cell culture in diagnostics, drug discovery, and biomedical research.

Instrumentation Demonstration

Hands-on experience with Laminar Flow Hood, CO₂ Incubator, Microscope, Centrifuge, Autoclave, and Water Bath.

Aseptic Techniques & Media Preparation

• Learn contamination-free handling under biosafety conditions.
• Prepare DMEM and RPMI-1640, adjust pH, and perform filtration and sterilization steps.

Tissue Processing & Cell Isolation

• Understand the Modified Tissue Processing Protocol for primary cell culture.
• Isolate cells from tissue samples and culture them under controlled environmental conditions.

Cell Staining & Microscopy

Perform Trypan Blue Viability Test, Hematoxylin & Eosin (H&E), MTT Assay, and Giemsa Staining to study cell morphology and health.

Cryopreservation & Cell Recovery

• Resuspension of Cryopreserved Cells: Thawing, gentle centrifugation, and plating.
• Subculturing & Cryopreservation: Passage cells and preserve them for long-term storage.

Quantitative Techniques

• Cell Counting & Viability: Using a hemocytometer and Trypan Blue exclusion.
• Cell Concentration Techniques: Compare gentle spin vs. high-speed centrifugation.
• Growth Media Comparison: Analyze cell performance in DMEM vs. RPMI-1640.

Primary Cell Culture Setup

• Preparation of tissue suspension and seeding of primary cells.
• Monitoring cell attachment, proliferation, and maintenance cycles.

Molecular Biology Integration

• Genomic DNA Isolation from cultured cells.
• Agarose Gel Electrophoresis and Visualization using Gel Documentation System — connecting cell culture to molecular diagnostics.

Lab Culture Exercise

Step-by-step practice of complete cell culture workflow — from tissue to molecular analysis.

Interactive Wrap-Up

Open discussion, troubleshooting, Q&A, feedback, and certificate distribution ceremony.

Outcome:
By the end of this training, participants will understand how to culture, monitor, and analyze cells — skills that form the foundation for careers in biotechnology, pharmacology, diagnostics, and molecular biology.

Program 2: Hematopoietic Stem Cell Culture

“Where Blood Becomes Biology — Exploring the Power of Regeneration”

Blood is more than just a fluid — it’s a universe of stem cells, immune cells, and regenerative potential. This specialized module introduces you to hematopoietic stem cells (HSCs) and their vital role in forming blood and immune systems.

What You’ll Learn

Fundamentals of Haematopoiesis

Understand the origin, differentiation, and function of hematopoietic stem cells (HSCs) and how they give rise to all blood cell lineages — red cells, white cells, and platelets.

Identification of Blood Cell Types: Develop the ability to recognize and classify different blood cells under the microscope using Romanowsky stains such as Leishman, Wright, and Giemsa.

Isolation of Hematopoietic Cells: Master multiple isolation techniques, including ACK lysis, Ficoll/Histopaque gradient centrifugation, EasySep, and Percoll density gradient, to separate mononuclear and stem cell populations from blood.

Hematopoietic Stem Cell Culture Setup: Learn how to establish and maintain primary HSC cultures from peripheral blood using DMEM/RPMI-1640 media enriched with FBS and antibiotics, under sterile and controlled CO₂ incubator conditions.

Cell Staining & Microscopic Analysis: Perform essential viability and morphological assessments using Trypan Blue, Hematoxylin & Eosin (H&E), MTT assay, and Giemsa staining to evaluate growth, health, and differentiation.

Media Preparation, Subculturing & Cryopreservation: Gain practical experience in formulating complete media, passaging suspension cells, and preserving hematopoietic cells for long-term storage using DMSO-based cryoprotectants.

Molecular Biology Skills: Isolate genomic DNA from cultured HSCs, run agarose gel electrophoresis, and visualize DNA using a gel documentation system, linking cellular and genetic analyses.

Data Analysis & Scientific Reporting: Analyze, interpret, and present experimental results — from cell counts to DNA profiles — and learn to prepare professional laboratory records, reports, and presentations for academic or research settings.

Summary:

“You’ll gain hands-on expertise in hematopoietic stem cell isolation, culture, staining, and molecular analysis — developing the core laboratory skills needed for research in stem cell biology, hematology, and regenerative medicine.”

Outcome:
Students gain firsthand experience with blood-derived stem cells, preparing them for advanced research in immunology, hematology, oncology, and regenerative medicine.

Why Join Herrick Healthcare’s Cell Culture Programs?

• Real laboratory exposure with modern infrastructure
• Expert guidance from research professionals
• Small, interactive batches (10–15 students)
• Certificate of Participation/Completion
• Opportunities for future internships and research collaborations

Program Information

• Venue: Herrick Healthcare Pvt. Ltd., Bhubaneswar
• Duration: [15 Days / 30 Days]
• Mode: Hands-On Laboratory Training
• Certification: Jointly issued by Herrick Healthcare & Heredity Biosciences

New Batch Starts Very Soon!

Seats are limited — reserve yours today to step into the world of real biomedical discovery.

For Registration & Queries

gopalpurohit@herrickhealthcare.com
+91-9437656000
 www.hereditybio.in
Herrick Healthcare Pvt. Ltd., Bhubaneswar

“Every great scientist once began with a single cell. Start your journey today — culture your skills, culture your future.”

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