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Unveil the intricate world of bacterial flagella through the Leifson method of staining. This step-by-step procedure ensures precise visualization of flagella distribution, providing valuable insights into bacterial motility. Let’s embark on this scientific journey:

1. Leifson Bacterial Flagella Stain
2. Microscope slides
3. Bunsen burner
4. Inoculating loop
5. Microorganisms with flagella
Procedure:

Materials:

(Complete procedure in the below video link)

1. Prepare a bacterial smear on a clean microscope slide using the inoculating loop.
2. Fix the smear by passing the slide over the Bunsen burner flame a few times.
3. Apply a few drops of Basic Fuchsin from the Leifson Stain Kit to cover the smear. Allow it to stand for 2-3 minutes.
4. Rinse the slide gently with distilled water to remove excess stain.
5. Apply a few drops of Tannic Acid solution from the kit for approximately 1 minute to enhance contrast.
6. Rinse the slide again with distilled water.
7. Counterstain the smear with Methylene Blue for 1 minute.
8. Rinse and blot the slide gently to remove excess stain.
9.Allow the slide to air dry completely.

Observation:
Examine the stained smear under a microscope. Flagella should appear as thin, thread-like structures extending from bacterial cells, revealing their distribution and arrangement.

Embark on this fascinating journey of bacterial exploration with the Leifson flagella staining method. Join us at Heredity Biosciences in our commitment to unravelling the mysteries of microbiology.

More experimental Activities: https://www.youtube.com/@hereditybioacademy7062

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