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Introduction:

0.9% Saline Solution, often referred to as Normal Saline, is a fundamental solution in medical and laboratory practices. It is an isotonic solution containing 9 grams of sodium chloride (NaCl) dissolved in distilled water to make 1 litter of solution. This concentration matches the osmolarity of human blood and is widely used for various applications, including intravenous fluid replacement and laboratory procedures.

Materials:

1. Sodium chloride (NaCl) – 9 grams
2. Distilled water – 1 liter
3. Sterile container or bottle
4. Stirring rod
5. Weighing balance
6. Autoclave (for sterilization)

Procedure:

1. Begin by measuring 9 grams of sodium chloride (NaCl) using a weighing balance. Ensure that the measurement is precise.
2. Pour the 9 grams of NaCl into a sterile container or bottle.
3. Add 1 liter of distilled water to the container with NaCl. Use caution to avoid contamination, and ensure that the water is at room temperature.
4. Gently stir the solution with a sterile stirring rod until all the sodium chloride has completely dissolved. This may take a few minutes.
5. Once the solution is prepared, it should be sterilized. The entire container or bottle, including its cap, should be autoclaved to ensure sterility.
6. After autoclaving, allow the solution to cool to room temperature.

Usage:

0.9% Saline Solution is now ready for use in medical procedures, laboratory experiments, or any application where an isotonic saline solution is required.

Note:

Always follow standard aseptic techniques and safety guidelines when preparing and handling saline solutions.

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